MOLECULAR HETEROGENEITY OF RIBONUCLEASE OF FAT TISSUE OF PATIENTS WITH COLORECTAL CANCER

Abstract

Summary. Aim: to investigate the molecular heterogeneity of ribonucleases (RNases) of adipose tissue in patients with colorectal cancer (CRC) with or without metastases. Objects and methods: the study was performed with adipose tissue obtained from patients with CRC who were being treated in the surgical department of the National Cancer Institute. Adipose tissue from CRC patients with metastases and without metastases was studied. To analyze the heterogeneity of RNases, the technique of enzymo- grams with quantitative assessment of activity in the GelAnalyzer program was used. Results: it was revealed that RNases of adipose tissue reveal significant molecular heterogeneity, which is more pronounced in patients with CRC with a metastatic process. From 2 to 6 isoforms of the enzyme are registered in the enzymograms of patients with metastases. The RNases activity in adipose tissue of patients with CRC with metastases is significantly higher than in patients without metastases. Conclusion: the obtained data can be used in the assessment of the course prognosis and the formation of therapeutic approaches to the treatment of patients with colorectal cancer.