ANTITUMOR EFFECTIVENESS OF THE COMBINED APPLICATION OF BACTERIA OF THE GENUS BIFIDOBACTERIUM AND B. SUBTILIS IMV B-7724 LECTIN

Abstract

Summary. Aim: to investigate of the antitumor effectiveness of the combined use of bacteria of the genus Bifidobacterium and the extracellular metabolite (lectin) of B. subtilis IMV B-7724. Object and methods: studies were performed on Balb/c mice (n = 52). Ehrlich adenocarcinoma was used as an experimental model. Starting from the 2nd day after the transplantation of tumor cells, the animals of the experimental groups were injected with lectin B. subtilis IMV B-7724 (s/c, 1 mg/kg of body weight, No. 10), B. animalis (per os, 7×105 CFU/ mouse, No. 10) or used their combination. The frequency of transplantation (%), the latent period of tumor emergence, the volume of tumors (mm3), and the life expectancy of animals were evaluated. Statistical processing of the results was carried out according to generally accepted methods of variational statistics. Results: administration of B. subtilis IMB B-7724 lectin (both alone and in combination with B. animalis) to mice with Ehrlich adenocarcinoma resulted in a significant inhibition of tumor growth. The tumor growth inhibition index was 55.0 and 50.5%, respectively. the animals of these groups were also characterized by a statistically significant increase in life expectancy compared to the control group: by 61.4 and 50.0%, respectively. The use of B. animalis alone did not have such a pronounced effect. Conclusion: on the Ehrlich adenocarcinoma model, a pronounced antitumor effect (inhibition of primary tumor growth, increase in life expectancy), both with the combined use of B. animalis and B. subtilis IMV B-7724 lectin, and with the separate administration of the bacterial lectin was demonstrated. The use of B. animalis alone did not affect the dynamics of tumor growth and the life expectancy of animals.