Study of Release of Biologically Active Compounds from Cord Blood Under Different Conditions of Low-Temperature Impact

Abstract

Here, we have studied the impact of cord blood destruction method on composition of the cord blood-derived low molecular fractions, and compared the cryodestruction with other methods of cell destruction before extracting. Human cord blood was destroyed by rapid or slow freezing / warming, hypotonic lysis and thermal destruction. The obtained substance was used to produce the cord blood fraction (CBF) by multi-stage ultrafiltration and lyophilization. Dry weight, CBF composition and total protein content in them were evaluated by chromatographic profiles (gel permeation and reverse-phase high-performance liquid chromatography). The CBFs, obtained by diff erent techniques for cord blood destruction were established to diff er in the content and molecular weights of the components. These fi ndings suggest the possibility to vary the amount and range of low molecular weight compounds in lyophilized cord blood fractions by using low temperatures and combining diff erent regimens of freezing / warming. Key words: cryodestruction, cord blood, biologically active substances, low-molecular fraction, low temperatures