COMBINED EFFECT OF ETHYLTHIOSULFАNYLATE AND VITAMIN E ON THE ENZYMATIC ACTIVITY OF AMINOTRANSFERASES IN RAT BLOOD PLASMA UNDER THE TOXIC EFFECT OF Cr(VI)

Abstract

Aim. The aim of our study was to investigate the effect of ethylthiosulfаnylate (ETS) and vitamin E on the enzymatic activity of blood plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of rats exposed to Cr(VI). Methods. The study was performed on male Wistar rats divided into 6 groups of 5 animals each. The animals were administrated with: group I (control) – physiological saline for 7 days; group III – 2.5 mg Cr(VI)/kg b.w. for 14 days; group II – oil for 14 days; group IV – vitamin E oil solution [20 mg/kg b.w.] for 14 days; group V – ETS oil solution [100 mg/kg b.w.] and vitamin E [20 mg/kg b.w.] for 14 days; group VI – ETS oil solution [100 mg/kg b.w.] and vitamin E [20 mg/kg b.w.] for 14 days, after which 2.5 mg Cr(VI)/kg b.w. for 14 days. We determined the activity of AST, ALT and de Ritis coefficient in blood plasma. Results: The activity of AST, ALT significantly increased (by 40%, 122%) and de Ritis coefficient significantly decreased (by 37%) in the blood plasma of rats after 14 days of Cr(VI) toxicity. AST, ALT increased (by 24%, 61%) also after combined exposure to ETS and vitamin E by subsequent 14-day exposure to Cr(VI) in the blood plasma of rats. Conclusion: Cr(VI)-induced stimulation of blood plasma AST and ALT was twofold lower after combined pretreatment with ETS and vitamin E.