Impact of feeding male rats F2 with different doses of germanium citrate on the content of traceelements in their tissues and organs

Abstract

Aim. To determine the impact of the dose of germanium citrate on the distribution of Fe, Zn, Cu, Co, Mn in tissues and organs of male F 2 rats. Methods. Physiological, biochemical, clinical, statistical. Results. It was established that there were changes in the content of Fe, Zn, Cu, Co, Mn in soft tissues and their distribution in liver, kidneys and lungs of male F 2 rats. It was demonstrated that these changes were mostly conditioned by organ-tissue specifi cities of functioning of some physiological systems of the organism, and less condition- ed – by the dose of germanium (10, 20 and 200 μg/kg of bodyweight). More vividly expressed changes in the content of these elements were established for kidneys at the effect of all the applied doses, and less express- ed – for liver and lungs. A positive impact of germanium citrate on the content of Cu, Co, Mn and Zn in the muscle tissues was determined at the effect of 10, 20 and 200 μg Ge, and as for Fe – 20 and 200 μg. The differences in the weight of liver, kidneys and lungs of rats of experimental and control groups were determined which eliminated the intergroup differences in the absolute content of the investigated microelements in liver, kidneys and lungs. The mentioned differences were more expressed for the absolute content of Cu in liver, Mn – in kidneys and lungs. Conclusions. Long-term introduction of germanium citrate into the organism of rats of generation F 2 with water in the amount of 10, 20 and 200 μg Ge/kg of the bodyweight is characterized by the changes in the content of Cu, Co, Mn, Fe, Zn both per one unit of soft tissue weight and their absolute content in the internal organs. The biological effect of germanium citrate is more expressed in the dose of 200 μg Ge/kg of the bodyweight, which conditions the increase in the content of Cu and Zn in all the investigated tissues of rats of this group at the background of the decreased level of Co in liver at the effect of 20 and 200 μg Ge, and at the effect of all the administered doses in kidneys and lungs. The level of Mn increased reliably in the muscle tissues of male F 2 rats at the effect of all the administered doses of Ge, Co 20 and 200 μg, Fe 10 and 20 μg, and Zn 10 and 200 μg Ge, which testifi es to the differences in the regulatory impact of NGeC on the level of investigated microelements in the muscle tissues of rats.