SNP analysis of Ukrainian maize inbreds with alternative state of molecular carotenogenesis marker crtRB1-3’TE

Abstract

Aim. SNP analysis and estimation of genetic relations in maize inbreds with the alternative state of β-carotene hy- droxylase 1 gene (crtRB1) by the molecular marker crtRB1-3ʹTE. Methods. SNP analysis, statistical methods. BDI- III panel with 384 SNP markers was used for comparative research of 35 maize inbreds developed and adopted in the northern Steppe of Ukraine. Results. SNP analysis of investigated inbred set showed that the part of dimorphic markers was 98.2 %, the average minor allele frequency (MAF) was 0.3040, the average polymorphism information content (PIC) was 0.3064, and the average shift of genetic diversity of markers was 0.3898. There was no significant difference between genetic SNP distances within both groups of inbreds with favourable/unfavourable alleles and between these groups by crtRB1-3ʹTE. The SNP distances were used to build a dendrogram of genetic relations be- tween maize inbreds with an alternative state of the crtRB1-3ʹTE marker. Conclusions. The relationship between the allelic state of the β-carotene hydroxylase 1 gene and single nucleotide polymorphism markers for maize inbreds is presented. The alleles of SNP markers BDI-III-130A, BDI-III-15A, BDI-III-60C, BDI-III-61C, BDI-III-116G, BDI- III-128A, and BDI-III-129A were found to be most frequent in the inbreds, which are carriers of the favourable allele of 543 bp of crtRB1 gene by the crtRB1-3ʹTE marker. The random distribution of alleles of this gene among maize inbreds developed in the northern Steppe of Ukraine was demonstrated.