Author:
Ramazanov Viktor, ,Volovelska Yelyzaveta,Bondarenko Valeriy, ,
Abstract
Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.
Publisher
National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka) (Publications)
Subject
Medicine (miscellaneous),Biophysics
Reference15 articles.
1. The cryopreservation of composite tissues: Principles and recent advancement on cryopreservation of different type of tissues;Bakhach;Organogenesis,2009
2. Very early posttraumatic serum alterations are significantly associated to initial massive RBC substitution, injury severity, multiple organ failure and adverse clinical outcome in multiple injured patients;Bogner;Eur J Med Res,2009
3. Osmotic effects of dilution on erythrocytes after freezing and thawing in glycerol-containing buffer;De Loecker;Cryobiology,1993
4. 4. Guchok VM, Vorotilin AM, Lougovov VI, Shrago MI. Therapeutic efficiency of RBC, cryopreserved under protection of a "Propanediosaccharole" preparation. Problems of Cryobiology. 1994; (4): 43-6.
5. Utilization and quality of cryopreserved red blood cells in transfusion medicine;Henkelman;Vox Sang,2015