Author:
Trotskyi P. A.,Shcherbak O. V.,Lyuta I. M.
Abstract
Aim. To study the effect of different concentrations of ethylene glycol and glycerin in equilibration and vitrifi cation
solutions on 1) the viability and further development of frozen-thawed bovine ovarian cumulus-oocyte complexes
(COCs), cryopreserved by vitrifi cation method, 2) on the effectiveness of inseminating mature oocytes, obtained from
them, and 3) on the formation of embryos. Methods. Biotechnological, cryobiological, morphological, cytogenetic,
and statistical methods, as well as methods of statistical data processing were used in the research. Results. The results
of experimental studies on the effect of different concentrations of ethylene glycol and glycerin in the equilibration
solution during cryopreservation of bovine ovarian COCs (n = 502) on their viability and further development after
freezing-thawing are presented. We also show the results of the comparative analysis of cryoresistant properties of
bovine ovarian COCs (n = 560) using different concentrations of ethylene glycol and glycerin, as cryoprotectants, in
the vitrifi cation solution in terms of the viability and maturation of the oocytes, which originated from these COCs,
up to metaphase II of meiosis. A comparative analysis of the application of ethylene glycol and glycerol in differ-
ent concentrations for the equilibration and vitrifi cation solutions in cryopreserving bovine ovarian COCs (n = 220)
demonstrated a relationship between the level of concentration of these cryoprotectants and the number of embryos
after in vitro insemination of mature gametes, obtained from these frozen-thawed COCs. Conclusions. It was found
that the use of 25 % ethylene glycol and 5 % glycerin in the equilibration solution and 10 % ethylene glycol and 40 %
glycerin in the vitrifi cation solution during cryopreservation of bovine ovarian COCs ensures lower toxicity of these
solutions and promotes more effi cient (up to 14.3 %) formation and development of embryos after in vitro insemina-
tion of mature gametes, obtained from these COCs
Publisher
National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka)
Cited by
1 articles.
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