Kinematic parameters and redox state of thawed ram sperm after adding nanocitrate of Mn, Zn, and Cu to the medium for cryopreservation

Abstract

The aim of the work was to find out the effect of adding nanocitrate of Mn, Zn and Cu to the medium for cryopreservation of ram sperm on kinematic indicators and respiratory activity of thawed sperm. The experiment was conducted on six clinically healthy breeder rams of the Texel breed aged 2–4 years. After receiving the ejaculates of the rams, they were evaluated for the volume, concentration and motility of the sperm and were divided into control and experimental groups. Control sperm samples were diluted with lactose-yolk-tris-citrate-glycerol medium (LYTCGM). Nanocitrates of trace elements were added to the medium in experimental samples of ram sperm in the following doses: Zn and Mn — 2.5, 5.0 and 7.5 μg/l, Cu — 1.25, 2.5 and 3.75 μg/l. Diluted sperm was packaged in straws, equilibrated for 2.5 hours and frozen. After thawing of sperm, motility, morphological damage of sperm, kinematic parameters of sperm motility (CASA), oxidation and reduction activity of sperm were determined. A dose-dependent effect of Mn, Zn, and Cu nanocitrates upon their addition to LYTCGM was established. The addition of Mn and Zn nanocitrate at a dose of 5.0 μg/l to LYTCGM significantly (P<0.05–0.01) increases the activity of thawed ram sperm, while the addition of Cu nanocitrate in increasing doses significantly reduces the motility of sperm in thawed ram sperm. Addition of Mn and Zn nanocitrate in an optimal dose of 5.0 μg/l to LYTCGM significantly (P<0.05–0.01) reduces the number of spermatozoa degenerated and with damaged acrosomes, and with the addition of Cu nanocitrate in increasing doses, morphological disorders of germ cell significantly increase cells The addition of Mn and Zn nanocitrate at a dose of 5.0 μg/l to LYTCGM significantly (P<0.01–0.001) increases the kinematic parameters of thawed ram sperm, and the addition of Cu nanocitrate in increasing doses significantly reduces the indicators of germ cell motility. The addition of Mn and Zn nanocitrates to the medium for cryopreservation of ram sperm increases the oxidation and inhibits the reduction activity of thawed sperm. Addition of Cu nanocitrate to LYTCGM in increasing doses reduces the oxidation and increases the reduction activity of thawed ram sperm.