The new technology of molecular and gene introduction method using discharge plasma: plasma brings features of random genome integration-free and damage-free to cells, genomic-DNA and external introducing molecules

Abstract

Abstract In the first half, this paper reviews the so-called “Plasma Gene Transfection” and “Plasma Molecular Introduction” which are the technology to introduce molecules such as plasmid DNA, proteins, and dye molecules into cells by discharge plasma treatment. In the latter half, the authors’ method with the micro discharge plasma is reviewed. This method induces the cell’s spontaneous uptake of external molecules by endocytosis which is triggered by the complex of weak stimuli produced by the micro discharge plasma treatment less than 10 ms and enables high introduction efficiency and high cell viability simultaneously. There is almost no damage to the cells and external introducing molecules, and there is almost no unintended integration of the introduced gene into the chromosome. The authors would like to call this feature “Random Genome Integration-Free.” This feature would enable safe and clean gene and molecular introduction for practical applications such as medicine and breeding.