Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing
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Published:2022-03-28
Issue:4
Volume:15
Page:042005
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ISSN:1882-0778
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Container-title:Applied Physics Express
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language:
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Short-container-title:Appl. Phys. Express
Author:
Ishikawa Tomohiro,Isobe Keisuke,Inazawa Kenta,Michikawa Takayuki,Namiki Kana,Miyawaki Atsushi,Kannari Fumihiko,Midorikawa Katsumi
Abstract
Abstract
Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μm multi-spots with an axial confinement of 5 μm, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μm.
Funder
RIKEN Junior Research Associate Program
Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering grant program
Japan Society for the Promotion of Science
Core Research for Evolutional Science and Technology
Subject
General Physics and Astronomy,General Engineering