Label-free visualization of cellular response to molecularly targeted agents using multiplex coherent anti-Stokes Raman scattering and third harmonic generation microscopy-Reference-Cited by-同舟云学术

Label-free visualization of cellular response to molecularly targeted agents using multiplex coherent anti-Stokes Raman scattering and third harmonic generation microscopy

Published:2022-09-09 Issue:10 Volume:15 Page:102001
ISSN:1882-0778
Container-title:Applied Physics Express
language:
Short-container-title:Appl. Phys. Express

Author:

Oka Yuki,Shen Larina Tzu-Wei,Mori Tomoko,Iwamura Takumi,Leproux Philippe,Matsusaka Satoshi,Kano Hideaki^ORCID

Abstract

Abstract Cellular responses to molecularly targeted agents (gefitinib and osimertinib) have been observed in cultured cancer cells using ultra-broadband multiplex coherent anti-Stokes Raman scattering and third harmonic generation microscopy. Lipid droplets (LDs) appeared in the cells 48 h after the exposure of cancer cells to gefitinib. This was also observed in the gefitinib-resistant strain, and the accumulation of LDs was significant. When exposed to osimertinib, LDs were not as pronounced as those with gefitinib. These agents act on cells through different mechanisms.

Funder

Japan Society for the Promotion of Science

Publisher

IOP Publishing

Subject

General Physics and Astronomy,General Engineering

Link

https://iopscience.iop.org/article/10.35848/1882-0786/ac8d47/pdf

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