Growth and development of potato microplants in vitro under the influence of bacterial metabolites isolated from permafrost

Abstract

The effects of permafrost bacterial strains on morphogenesis, rhizogenesis, and photosynthesis in potato micro-plants under in vitro conditions were assessed from 2019 to 2021. We used three bacterial culture strains from the collection of the Cryosphere Bioresources Department, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences. The study was conducted on microgrowers of three potato varieties: Zhukovsky Early, Rozara and Red Scarlett. Two strains of bacteria were isolated from the cores of perennially frozen rocks. We found that the combined cultivation of potato microgrowers under in vitro conditions on Murasige-Skug nutrient medium with secondary metabolites of the bacterial strains Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2, introduced at the time of cutting at a dose of 250 µL, had the greatest stimulatory effect on its morphogenesis, rhizogenesis, and photosynthesis. Thus, the proposed cultivation method accelerates clonal micropropropagation of in vitro materials for original potato seed production.