A Comparative Study of Antibacterial Activity of Leaf and Root Extracts of Sida acuta Burm. F. Against Staphylococcus sciuri Isolated From Acne Using Computer-Aided Technique

Abstract

This study investigated the significant anti-bacteriological efficacy between leaf and root of Sida acuta Burm. F. extracts against acne inducing bacteria. Twelve samples were collected from twelve persons with facial acne. Nutrient agar was used for the isolation of the bacteria and 16S rRNA gene sequencing technique was used for molecular identification. Bioinformatics analysis was carried out using Basic Local Alignment Search Tools (BLAST) for similarity, Cluster W software for multiple sequence alignment and MEGA 7 software for the phylogenetic analysis. Antibacterial susceptibility test of the bacterial isolates was carried out using the agar well diffusion method. A total number of six bacterial species were identified as Staphylococcus sciuri. The study revealed that control antibiotic- Ciprofloxacin (CFX) inhibited the growth of all the bacterial isolates. Staphylococcus sciuri was susceptible to leaf ethanol extract of Sida acuta at 75 μg/ml and 100 μg/ml and was susceptible to the aqueous root extract at 25 μg/ml and 100 μg/ml while it was susceptible to the ethanol root extract at 2.5 μg/ml. From this study, it was shown that the aqueous root extracts had more zones of inhibition with the maximum inhibition of 45mm at 100 μg/ml. Hence, Sida acuta Burm. F. is a powerful antimicrobial agent.