Evaluation of the microorganisms growth with stabilized doxorubicin on nano graphene oxide substrate

Abstract

In order to measure the effect of graphene oxide-stabilized drug on the growth of microorganisms, first the graphene oxide used was prepared from graphite modified by Homers method. After preparation of graphene carrier, doxorubicin was loaded by ultra sound. Then, due to the high biocompatibility of drug carriers, their cytotoxicity and antibacterial properties were determined by the methods of minimum inhibitory concentration, minimum bactericidal concentration and plate penetration. The effect of prepared carriers containing metal nanoparticles on the growth of microorganisms such Enterococcus, Corynebacterium and Escherichia coli were studied as statistical means. The best minimum inhibitory concentration (MIC) values were obtained for Fe/Ag/M (rGO/Cis) nanoparticles, which had a lower minimum concentration compared to other nanoparticles or their combinations. Among these, the rGO/Cis sample had the best performance. Bacteria of Corynebacterium and Enterococcus showed the greatest reduction in viability against RGO / Cis up to 125 and 62.5 μg/mL, respectively. Carriers prepared in this study were generally not very toxic, as a high percentage of cells survived in the concentration range less than 100 μg/mL.