Author:
Mate Toth,Zoltan Szabo,Zoltan Toth
Abstract
Capsicum (pepper) species have high economic values as vegetable crops and medicinal plants. Most of the Capsicum is known to be recalcitrant to plant regeneration in vitro, and to genetic transformation with Agrobacterium tumefaciens. However, genetic improvement against pathogens requires discovering new pest resistance genes and revealing their functions and mechanism in vitro. The development of improved transformation methods serves this purpose, which needs a binary vector technology carrying the gene of interest to be transferred into the host plants. Agrobacterium rhizogenes mediated transformation serves as a useful alternative way for the Capsicum transformation. The A. rhizogenes transformation compared to the A. tumefaciens transformation has the advantage that the method needs no regeneration step in vitro. Our goal is to obtain a highly efficient transformation system that can be used to study the functions of different genes in Capsicum annuum varieties. Our study’s further goal is to validate and describe the candidate gene (Me1) involved in resistance against root-knot nematode species.
Publisher
Heighten Science Publications Corporation
Reference14 articles.
1. ) genotypes;Hasan;Int J Agric Res Innovat Technol,2014
2. 2. Wolf D, Matzevitch T, Steinitz B, Zelcer A. Why is it difficult to obtain transgenic pepper plants? Acta Horticulturae. 2001; 560: 229-233.
3. );Li;Plant Cell Rep,2003
4. 4. Steinitz B, Wolf D, Matzevitch-Josef T, Zelcer A. Regeneration in vitro and genetic transformation of pepper (Capsicum spp.): The current state of art. Capsicum and Eggplant Newsletter.1999; 18: 9-15.
5. 5. Setamam N Md, Sidik NJ, Rahman ZA, Zai CRCM. Induction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants. BMC Res Notes. 2014; 7: 1-8. PubMed: https://pubmed.ncbi.nlm.nih.gov/24981787/
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