A SIMPLE STAINING TECHNIQUE FOR ASSESSING FEEDING DAMAGE BY LEPTOGLOSSUS OCCIDENTALIS HEIDEMANN (HEMIPTERA: COREIDAE) ON CONES-Reference-Cited by-同舟云学术

A SIMPLE STAINING TECHNIQUE FOR ASSESSING FEEDING DAMAGE BY LEPTOGLOSSUS OCCIDENTALIS HEIDEMANN (HEMIPTERA: COREIDAE) ON CONES

Published:1990-10 Issue:5 Volume:122 Page:963-968
ISSN:0008-347X
Container-title:The Canadian Entomologist
language:en
Short-container-title:Can Entomol

Author:

Campbell Bruce C.,Shea Patrick J.

Abstract

AbstractPectinmethylesterase (PME) activity was found in the salivary glands of nymphs and adults of a leaf-footed bug, Leptoglossus occidentalis Heidemann. Puncture wounds in cone scales resulting from PME activity in the saliva of these bugs were seen by staining with a 0.05% aqueous solution of ruthenium red. This staining technique can be used to estimate feeding damage by L. occidentalis on cones of western white pine (Pinus monticola Dougl.), sugar pine (P. lambertiana Dougl.), and Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco).

Publisher

Cambridge University Press (CUP)

Subject

Insect Science,Molecular Biology,Physiology,Ecology, Evolution, Behavior and Systematics,Structural Biology

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