AN EXTRACTION METHOD FOR RAPID COUNTS OF INSECT EGGS AND SMALL ORGANISMS

Author:

Condrashoff S. F.

Abstract

AbstractInsect eggs and small organisms may be removed from foliage or bark with solvents and recovered on filter paper. Tests with Acleris variana (Fern.) showed that 96 per cent of eggs were recovered compared to 47–70 per cent counted on foliage. Tests indicated that the extraction method required one-third to one-tenth the time spent in counting the organisms on foliage. The extraction method was more consistent than counts on foliage, and less affected by differences in personnel and working conditions. The method and the solvent must be selected and modified to suit the organism.

Publisher

Cambridge University Press (CUP)

Subject

Insect Science,Molecular Biology,Physiology,Ecology, Evolution, Behavior and Systematics,Structural Biology

Reference5 articles.

1. A method for sampling eggs of the black-headed budworm;Silver;J. Forestry,1959

2. Modification of the centrifugal-flotation technique for the isolation and concentration of nematodes and their eggs from soil and plant tissue;Coveness;Proc. Helminthol. Soc. Wash.,1955

3. Defoliation of western hemlock by a needle miner, Epinotia sp;Condrashoff;Bi-mon. Prog. Rep.,1966

4. Flotation Technique for Extracting Eggs of Diabrotica spp. and Other Organisms from Soil

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