Activity of Markers of Bone Metabolism in Animals with Simulated Osteoporosis after Dental Implantation

Abstract

The purpose of the study was to evaluate the state of bone metabolism by determining the activity of alkaline phosphatase and tartrate-resistant acid phosphatase in the blood of experimental animals with simulated osteoporosis after dental implantation under the influence of corrective osteotropic therapy. Materials and methods. The experimental part of the study was performed on 78 rabbits (Chinchilla breed): 15 animals – the control group and 63 animals – the experimental group (31 females and 32 males). After placement of implants, in the blood of the experimental animals, after 1, 3, 6 and 12 months, the activity of alkaline phosphatase was determined by a unified colorimetric method and the activity of tartrate-resistant acid phosphatase – by an immunoenzymatic method using the “Bone TRACP Assay” kit by Assay Pro (USA). Results and discussion. On the 12th month of the experimental research in the experimental animals of subgroup A (I), the activity of alkaline phosphatase in the blood was significantly lower compared to the data of intact animals, p<0.01 and 64.71% lower, compared to the initial data, p1<0.01. It was noted that after 12 months of observation in animals of subgroup B (I), the activity of alkaline phosphatase in the blood was equal to the data of intact animals, p>0.05, and was 72.06% lower than the initial values, p1<0.01. After 12 months of research, the animals of the subgroups, where no correction of simulated osteoporosis was performed, observed an intense increase in the activity of tartrate-resistant acid phosphatase in the blood. At the same time, in the subgroups where the medicinal treatment of osteoporotic phenomena was carried out, there was a tendency to decrease the activity of tartrate-resistant acid phosphatase in the blood, the data of which after 12 months of observation were equal to the values in intact animals, p>0.05, and were probably lower than the initial values. Thus, a decrease in tartrate-resistant acid phosphatase in the blood of animals with simulated osteoporosis treated with antiresorptive therapy can be interpreted as a decrease in bone tissue resorption. Conclusion. Summarizing the results of these studies and paying attention to the results of the activity of alkaline phosphatase and tartrate-resistant acid phosphatase in the blood of animals with simulated osteoporosis, it can be assumed that in animals that received drug therapy aimed at the correction of osteoporotic phenomena, the processes of bone material synthesis prevail over resorption