Influence of Cell Therapy on the Dynamics of Inflammatory Markers in Blood of Rats with Adjuvant Arthritis

Abstract

The purpose of the study was to investigate the dynamics of inflammation in blood of the rats with adjuvant arthritis under the conditions of generalized and local administration of cryopreserved multipotent mesenchymal stromal cells from adipose and cartilage tissues. Materials and methods. A model of adjuvant arthritis in male rats was induced by subplantar administration of Freund’s complete adjuvant. On the 7th day of modeling, experimental animals were administered: control group – saline; experimental group 1 and 2 – cryopreserved multipotent mesenchymal stromal cells from adipose or cartilaginous tissue locally; experimental group 3 and 4 – cryopreserved multipotent mesenchymal stromal cells from adipose or cartilaginous tissue generalized. Groups of intact animals served as control. On days 14, 21 and 28 erythrocyte sedimentation rate was determined in blood, and content of total protein, C-reactive protein and rheumatoid factor were measured in serum. Results and discussion. In the control group of animals, the inflammatory process was pronounced, as evidenced by a significant increase in the studied parameters throughout the observation period. The use of cryopreserved multipotent mesenchymal stromal cells from adipose and cartilaginous tissues led to the restoration of the total protein content and erythrocyte sedimentation rate on the 28th day of observation, as well as positive dynamics in the level of C-reactive protein and rheumatoid factor. Generalized administration of cells had a more pronounced therapeutic effect compared to the animals with local one. A comparative assessment of the use of cryopreserved multipotent mesenchymal stromal cells from adipose and cartilaginous tissues showed the absence of significant changes in the studied parameters both with studied ways of cell administration. These data can be used to justify and develop methods of treating arthritis in clinical practice. But important issue that needs further research is the determination of cell immunity in the animals with adjuvant arthritis and treatment of cryopreserved multipotent mesenchymal stromal cells from adipose and cartilage tissues. Conclusion. Cell therapy with the use of cryopreserved multipotent mesenchymal stromal cells from the investigated sources provided by both local and generalized administration to animals with adjuvant arthritis has a modulating effect on the course of inflammation