Evaluation of Rats’ Spermatogenesis State under Irradiation Exposure and Influence of Natural Origin Drugs

Abstract

Male infertility is probably to be affected by intense heat exposure and extreme exposure to pesticides, irradiation, electromagnetic radiation and other hazardous substances. The authors of most of the works agree that the cells of the basement membrane of the tubules and spermatogonia, which are quickly exhausted after irradiation, are the most vulnerable in the testes. The type and extent of these changes depends on the dose, duration and frequency of radiation. The participation of connective tissue in almost all typical pathological conditions has been shown: inflammation, allergy, carcinogenesis, hypoxia, free radical oxidation processes, etc. The purpose of the work was to study evaluation the state of rats' spermatogenesis under conditions of the total irradiation exposure and to correct the pathological process with drugs of natural origin Chondroitin sulfate and "Bioglobin-U". Material and methods. A model of total single irradiation with a dose of 3 Gy was used to obtain the pathology of the testes, after which the substance Chondroitin sulfate was administered at a dose of 60 mg / kg; comparison drug Tribestan at a dose of 60 mg/kg; the drug "Bioglobin-U" at a dose of 200 μl / kg and Chondroitin sulfate + Tribestan in the above doses. Results and discussion. 30 days of exposure of single total irradiation with a dose of 3 Gy led to significant disruption of the morphological state of the testes and spermatogenic epithelium of rats. Thus, in rats with irradiation, the number of normal spermatogonia in the convoluted tubules was reduced by 5.7 times, there were practically no convoluted tubules with spermatocytes in the metaphase of the 2nd maturation division (with the 12th stage of meiosis) and tubules with desquamated spermatogenic epithelium, the index of spermatogenesis decreased by almost 6.5 times. The introduction of natural origin drugs led to an improvement in the quantitative and qualitative indicators of spermatogenesis. Conclusion. In the conditions of irradiation exposure, Chondroitin sulfate has more significant regenerative properties, the effect of which is almost 1.9 times better than the effect of the reference drug Tribestan. The effectiveness of "Bioglobin-U" in these experimental conditions was somewhat inferior to Chondroitin sulfate, but this drug was almost 1.4 times more effective than the reference drug Tribestan. The combination of Tribestan and the substance Chondroitin sulfate was more than 1.4 times more effective than the reference drug Tribestan itself