In vitro DNA Binding Interaction Studies of Hydrazones and Thiosemicarbazones Derived from Pyrazole Moiety

Abstract

In this work, the novel hydrazones and thiosemicarbazones derived from pyrazole moieties were synthesized by reacting 4-chloro-1-methyl-1H-pyrzolecarboxaldehyde with various hydrazides and thiosemicarbazides. All the synthesized compounds were characterized and confirmed with different spectroscopic techniques like FTIR, 1H NMR and LC-MS. The ct-DNA binding interaction studies were evaluated by using UV-visible spectroscopic and fluorescence spectrophotometric techniques with calf thymus DNA. The obtained binding constants of the synthesized were found to be 7 [8.44 × 104] > 4 [3.61 × 104] > 2 [3.60 × 104] > 1 [3.18 × 104] > 8 [2.85 × 104 M-1].