Development of Chitosan-Collagen/Substituted Hydroxyapatite-Polypyrrole Biocomposite with Prospective Application in Bone Tissue Engineering Scaffolds

Abstract

An innovative hybrid based on chitosan-collagen/copper, manganese substituted hydroxyapatitepolypyrrole (CS-COL/CMHA-PPY) was developed in present study to further enhance the surface morphology of regenerative medicine scaffolds by combining the synthesized copper, manganese substituted hydroxyapatite (CMHA) and polypyrrole with the chitosan and collage solution accompanied by solvent casting techniques. The fabricated biocomposite was characterized using FTIR, XRD and SEM techniques. It investigated the effects of CMHA and polypyrrole on the scaffold's physico-chemical characteristics like swelling proportion, degradation, and mechanical nature. The CS-COL/CMHA-PPY biocomposite demonstrated lower deterioration rate and higher mechanical properties according to the chitosan-collagen and CS-COL/CMHA biocomposite. MTT assay conducted a tentative evaluation of the tissue engineering and cytotoxicity of the chitosan-biocomposite scaffold utilizing osteoblast cells. Tests demonstrated no toxicity, so osteoblast cells were bound to the biocomposite pore surfaces so propagated. Such results indicated that the scaffold established has the preconditions which can be used as a scaffold for the reconstruction of bone tissue.