Polyaniline based Modified Sensor with Silver Nanoparticles for Detection of Urea

Abstract

A novel polyaniline (PANI) based amperometric urea biosensor developed for selective and quantitative sensing of urea using mediator enzyme urease on graphite paste electrode modified with silver nanoparticle (Ag) (PANI/Gr/AgNO3) and without silver nanoparticle(PANI/Gr) and measure the amperometric response caused by the immobilized urease reaction system. Urease immobilization on electrode was investigated using an amperometric method and factors like pH after its immobilization discussed. Prepared materials were characterized by analytical techniques such as ultra violet visible and SEM analysis. The performance of the developed urea biosensor was evaluated and obtained urea biosensor exhibited shorter response time (3 s), wider linear range, lower detection limit and good stability with about 90% of the original response signal retained after 2 months for silver nanoparticle.