Affiliation:
1. Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram, Mataram 83125, Indonesia
Abstract
This study aimed to separate fatty acid ethanolamides (FAE) through an enzymatic reaction of
ethanolamine and the Terminalia catappa (Local name: Ketapang) seed oil by using reverse-phase
high-performance liquid chromatography (RP-HPLC). A 4.6 mm × 150 mm, 5 μm SunFire C18 column
was used. Separation optimization was performed by analyzing different variables, which includes
mobile phase composition (acetonitrile/water), flow rate and sample concentration. The separation
results showed that the retention time for all components was 4.5; 2.4; 12.9; and 6.3 min for fatty acid
ethanolamides: oleoylethanolamide, linoleoylethanolamide, stearyl diethanolamide and
palmitoylethanolamide, respectively. Inter-peak resolution of >1.5 indicated that each component was
accurately separated.
Publisher
Asian Journal of Chemistry