Affiliation:
1. Division of Chemistry, Department of Science & Humanities, Vignan’s Foundation for Science, Technology & Research (Deemed to be University), Vadlamudi-522213, India
Abstract
An RP-HPLC method is validated to separate and quantify flubendiamide and its relevant eight related
impurities. The ideal chromatographic separation of flubendiamide and its eight allied impurities was
performed on Ace-5 C-18 column (5 μm, 25 cm × 4.6 mm) with formic acid (0.1%) in Milli-Q water
plus acetonitrile in a gradient elution at a flowing rate of 1.2 mL/min as mobile phase. Photodiode array
based detection at wavelength of 240 nm was deployed to monitor flubendiamide and its eight related
impurities. The robustness, accuracy, linearity, precision and detection and quantitation limitations of
the new RP-HPLC technique for flubendiamide and its eight related impurities were all validated.
Both FDE and eight process allied impurities had quantitation limits in the range of 0.07 to 0.12
μg/mL. This new HPLC technique was successfully applied to ascertain the purity of flubendiamide
analyte and levels of eight related impurities in five bulk lot products of flubendiamide synthesized in
the laboratory. The present study also deals with the bulk batch synthesis of flubendiamide and
characterization of flubendiamide and its relevant eight impurities by 1H NMR, ESI-MS and IR
spectroscopy.
Publisher
Asian Journal of Chemistry
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