Synthesis, Molecular Docking Studies, Antimicrobial, Anticancer and Antioxidant Activity of Some Novel Mannich Bases of Isatin Scaffold

Abstract

A series of novel Mannich bases of isatin derivatives (VIIIa-VIIIt) was synthesized and evaluated as potential antimicrobial, antioxidant, anticancer activities and molecular docking studies. Structure of all the isatin derivatives was evaluated by IR, 1H NMR and mass spectral analysis. The antimicrobial activity results indicated that compounds VIIIb, VIIIi, VIIIm and VIIIo showed good activity in comparison to the activities of the standard molecules. Further, all isatin derivatives (VIIIa-VIIIt) have studied for their antioxidant activity by using ferric reducing antioxidant power assay (FRAP) method. Most of the synthesized compounds exhibited the significant antioxidant activities. The anticancer activity results suggested that the isatin derivatives VIIIm and VIIIg show the more activity against MCF-7 cells in comparison with doxorubicin as standard drug. Furthermore, the molecular docking studies of Mannich bases of isatin derivatives showed good agreement with the biological results when their binding pattern and affinity towards the active site of EGFR was inquisition.