Green Synthesis and in vitro Anti-Tubercular Activity of Monometallic and Bimetallic Oxide Nanoparticles

Abstract

Tuberculosis (TB) is an infectious disease with limited drugs and currently the research is focused on its prevention, diagnosis and treatment. In recent studies, it is observed that the therapeutics approach of tuberculosis is more convenient to use nanoparticles for the treatment. With this regard, we foccussed present study towards modified green synthesis of monometallic oxide and bimetallic Cu-Zn and Cu-Mn oxide nanoparticles using Ficus religosa extract. All the synthesized bimetallic Cu-Zn oxide and Cu-Mn oxide nanoparticles were characterized by powdered XRD, FTIR, FE-SEM with EDS reports and ESR spectra. All the monometallic and bimetallic oxide nanoparticles were tested for in vitro anti-tubercular, antibacterial and cytotoxic activity. The Cu-Mn oxide nanoparticles showed promising result with IC50 = 6.27 μg/mL, MIC = 53.61 μg/mL and IC50 = 8.81 μg/mL, MIC= 54.82 μg/mL against M. tuberculosis H37Ra at dormant state and active state, respectively. Whereas Cu-Zn and Cu-Mn oxide nanoparticles showed moderate effect against M. tuberculosis H37Ra. On the other hand, all these nanoparticles were inactive against two Gram-positive and two Gram-negative bacterial strains with low cytotoxicity.