A Simple and Reliable RPLC Method for Simultaneous Determination of Five β-Blocker Drugs in Pharmaceuticals and Human Plasma

Author:

Albishri Hassan M.1,Alshammari Basmah H1,El-Hady Deia Abd2ORCID

Affiliation:

1. Chemistry Department, Faculty of Science, King Abdulaziz University, Solimania, 80203 Jeddah, Saudi Arabia

2. Chemistry Department, Faculty of Science, University of Jeddah, Osfan Road, P.O. Box 80327, 21589 Jeddah, Saudi Arabia

Abstract

A simple and reliable simultaneous determination of β-blocker drugs (acebutolol, pindolol, atenolol, nadolol and oxprenolol) was accomplished by reversed phase liquid chromatography-ultraviolet detection (RPLC/UV). The chromatographic separation was achieved on a SB-C18 ZORBAX® column (250 mm × 4.6 mm i.d., 5 micron), using a mobile phase consisted of 0.02 mol L-1 phosphate buffer (pH = 3.5)-acetonitrile (70:30, v/v). Isocratic elution was used at a flow rate of 1.0 mL min-1. The UV detector was operated at 230 nm and the column temperature was maintained at 30 °C. Under optimal conditions, a good linearity in the range of 10-500 μg L–1 for five β-blocker drugs with the correlation of determinations (R2) higher than 0.9971 was achieved. The proposed method was successfully applied to routine analysis of several β-blockers in pharmaceutical tablets. Moreover, the results obtained from this study demonstrated that the validated method can be successfully used to routine analyze the therapeutic concentrations of several β-blockers in human plasma. The LOD and LOQ for selected β-blockers were ranged within 0.050-0.538 and 0.152-1.794 μg L-1 & 0.192-0.845 and 0.641-2.562 μg L-1, respectively, for pharmaceutical and plasma samples. Results of intra-day and inter-day precision expressed in terms of %RSD were found to be less than 2.0. Accuracy of the RPLC method was assessed by performing replicate analyses of selected β-blockers in samples against a calibration curve indicating high recoveries within 96.7-110.5%. This new method could be used for analysis of large sample series of five β-blockers in routine laboratory work.

Publisher

Asian Journal of Chemistry

Subject

General Chemistry

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