Life-history features and oceanography drive phylogeographic patterns of the chitonAcanthochitonacf.rubrolineata(Lischke, 1873) in the northwestern Pacific

Abstract

Chitons are a group of marine mollusks (class Polyplacophora) characterized by having eight articulating shell plates on their dorsal body surface. They represent suitable materials for studying the spatiotemporal processes that underlie population differentiation and speciation in ocean environments. Here we performed population genetic analyses on the northwestern Pacific chitonAcanthochitonacf.rubrolineata(Lischke, 1873) using two mitochondrial gene fragments (COI and 16S) from 180 individuals sampled from 11 populations among the coastal waters of Korea, Japan, and China. The phylogenetic network uncovered a reticulated relationship with several sub-haplogroups for allA.cf.rubrolineatahaplotypes. SAMOVA analyses suggested the best grouping occurred at three groups (ΦCT = 0.151,P < 0.0001), which geographically corresponds to hydrographic discontinuity among the coastal regions of Korea, Japan, and China. The assumed limited dispersal ability ofA.cf.rubrolineata, coupled with northeasterly flowing, trifurcate warm currents, might have contributed to the genetic differentiation among the three groups. Meanwhile, a high level of within-group genetic homogeneity was detected, indicating extensive coastal currents might facilitate gene flow among the populations within each group. Bayesian skyline plots demonstrated significant population expansion after the Last Glacial Period (110-25 thousand years ago) for all studied populations except the Japan group. Together these results suggest that the present-day phylogeographic patterns ofA.cf.rubrolineataare strongly affected by the interplay of historical and/or contemporary oceanography and species-specific life-history features.