Affiliation:
1. College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
2. Research Center of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China
Abstract
Background
Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing.
Methods
In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants.
Results
Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.
Funder
National Key R&D Program of China
National Natural Science Foundation of China
Key-construction Subject Plan of Anhui Province
National Key Research and Development Program of China
Anhui Provincial Natural Science Fund
Key Project of Natural Science Research of Anhui Provincial Education Department
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience