Evaluation of sugarcane promising clones based on the morphophysiological traits developed from fuzz
Author:
Saleem Bilal1, Uzair Muhammad1, Noman Muhammad12, Attia Kotb A.3, Zhang Muqing4, Alwahaibi Mona S.5, Zahra Nageen1, Naeem Muhammad Kashif1, Mohammed Arif A.3, Fiaz Sajid6, Kimiko Itoh7, Khan Muhammad Ramzan1
Affiliation:
1. Functional Genomics and Bioinformatics Lab, National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan 2. Plant Molecular Physiology Laboratory, Department of Biology, Federal University of Lavras, Lavras, Minas Gerais, Brazil 3. Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia 4. Guangxi Key Lab for Sugarcane Biology, State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, College of Agriculture, Guangxi University, Nanning, China 5. Department of Botany and Microbiology, College of Science, King Saud University, Al-Riyadh, Saudi Arabia 6. Plant Breeding and Genetics, The University of Haripur, Haripur, Pakistan 7. Institute of Science and Technology, Niigata University, Niigata, Japan
Abstract
Sugarcane is one of the critical commercial crops and principal sources of ethanol and sugar worldwide. Unfavorable conditions and poor seed setting rates hinder variety development in sugarcane. Countries like Pakistan directly import fuzz (true seed) and other propagation material from the USA, China, Brazil, etc. In this study, we imported fuzz from China, developed 29 genotypes germinating in the glasshouse, and evaluated at field conditions along with two local checks (CPF-251 and HSF-240). Morphophysiological data were recorded, including plant height (PH), cane length (CL), internodal length (IL), tiller number (TN), brix percentage (B), cane diameter (CD), chlorophyll a (Chl. a), chlorophyll b (Chl. b), and total chlorophyll (T. Chl). Results showed highly significant (p < 0.001) differences among the sugarcane accessions for all the studied traits. High broad-sense heritability (81.89% to 99.91%) was recorded for all the studied parameters. Genetic Advance (GA) ranges from 4.6% to 65.32%. The highest GA was observed for PH (65.32%), followed by CL (63.28%). Chlorophyll leaching assay was also performed at different time points (0, 50, 100, 150, and 200 min). All the genotypes showed the same leaching trend at all times, and better performing genotypes showed less leaching compared to poor performing, indicating the high amount of cutin and wax on the leaf surface. Correlation analysis showed that PH, CL, IL, and TN had significant associations. Principal components analysis (PCA) further confirms these results. Based on PCA and correlation results, PH, CL, IL, and TN can be utilized as a selection criterion for sugarcane improvement. Genotypes such as NS-4a, NS-5, NS-6, NS-8, NS-9, and NS-15 are recommended for future breeding programs related to sugarcane variety development.
Funder
Researchers Supporting Project King Saud University, Riyadh, Saudi Arabia The PSDP and the Ministry of National Food Security and Research Sugarcane Research Institute, Faisalabad The Guangxi Key Lab for Sugarcane Biology, Nanning, China Ministry of National Food Security and Research of Pakistan Sino-Pak Agricultural Breeding Innovations Project
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience
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