Freshwater sponge hosts and their green algae symbionts: a tractable model to understand intracellular symbiosis

Author:

Hall Chelsea12,Camilli Sara13,Dwaah Henry1,Kornegay Benjamin1,Lacy Christie1,Hill Malcolm S.14,Hill April L.14

Affiliation:

1. Biology, University of Richmond, Richmond, VA, United States of America

2. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Charlottesville, VA, USA

3. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, United States of America

4. Biology, Bates College, Lewiston, ME, United States of America

Abstract

In many freshwater habitats, green algae form intracellular symbioses with a variety of heterotrophic host taxa including several species of freshwater sponge. These sponges perform important ecological roles in their habitats, and the poriferan:green algae partnerships offers unique opportunities to study the evolutionary origins and ecological persistence of endosymbioses. We examined the association betweenEphydatia muelleriand its chlorophyte partner to identify features of host cellular and genetic responses to the presence of intracellular algal partners.Chlorella-like green algal symbionts were isolated from field-collected adultE. muelleritissue harboring algae. The sponge-derived algae were successfully cultured and subsequently used to reinfect aposymbioticE. muelleritissue. We used confocal microscopy to follow the fate of the sponge-derived algae after inoculating algae-freeE. muellerigrown from gemmules to show temporal patterns of symbiont location within host tissue. We also infected aposymbioticE. muelleriwith sponge-derived algae, and performed RNASeq to study differential expression patterns in the host relative to symbiotic states. We compare and contrast our findings with work in other systems (e.g., endosymbioticHydra) to explore possible conserved evolutionary pathways that may lead to stable mutualistic endosymbioses. Our work demonstrates that freshwater sponges offer many tractable qualities to study features of intracellular occupancy and thus meet criteria desired for a model system.

Funder

National Science Foundation

Institutional Development Award

National Institute of General Medical Sciences of the National Institutes of Health

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3