Affiliation:
1. Institute of Parasitology, McGill University, Montreal, Canada
2. Department of Animal Science, McGill University, Montreal, Quebec, Canada
Abstract
Engineered nanoparticles are used at an increasing rate in both industry and medicine without fully understanding their impact on health and environment. The nematodeCaenorhabditis elegansis a suitable model to study the toxic effects of nanoparticles as it is amenable to comprehensive phenotyping, such as locomotion, growth, neurotoxicity and reproduction. In this study, we systematically evaluated the effects of silver (Ag) and five metal oxide nanoparticles: SiO2, CeO2, CuO, Al2O3and TiO2. The results showed that Ag and SiO2exposures had the most toxic effects on locomotion velocity, growth and reproduction, whereas CeO2, Al2O3and CuO exposures were mostly neurotoxic. We further performed RNAseq to compare the gene expression profiles underlying Ag and SiO2toxicities. Gene set enrichment analyses revealed that exposures to Ag and SiO2consistently downregulated several biological processes (regulations in locomotion, reproductive process and cell growth) and pathways (neuroactive ligand-receptor interaction, wnt and MAPK signaling, etc.), with opposite effects on genes involved in innate immunity. Our results contribute to mechanistic insights into toxicity of Ag and SiO2nanoparticles and demonstrated thatC. elegansas a valuable model for nanotoxicity assessment.
Funder
Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT), Genome Canada, Genome Quebec
The Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience
Cited by
12 articles.
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