Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages

Author:

Kaewseekhao Benjawan12,Roytrakul Sittiruk3,Yingchutrakul Yodying3,Laohaviroj Marut1,Salao Kanin1,Faksri Kiatichai12

Affiliation:

1. Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

2. Research and Diagnostic Center for Emerging Infectious Diseases (RCEID), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

3. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand

Abstract

Background Differences in immune responses against different lineages of Mycobacterium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune responses were also investigated during infection and when the bacilli had been eliminated from the immune cells. Methods Human primary leukocytes were infected with strains representing three lineages of Mtb (East-Asian, Indo-Oceanic and Euro-American). Label-free GeLC MS/MS proteomic analysis of secretomes was performed. The response of each immune-cell type was compared with the appropriate interactome database for each. Results The expression pattern of proteins secreted by Mtb-infected leukocytes differed among Mtb lineages. The ancestral lineage (IO lineage) had a greater ability to activate MMP14 (associated with leukocyte migration) than did the more recent lineages (EA and EuA). During infection, proteins secreted by macrophages, dendritic cells, neutrophils and B-cells were associated with cell proliferation. Following clearance of Mtb, proteins associated with interferon signaling were found in macrophages, dendritic cells and neutrophils: proteins associated with antigen processing were found in B-cells and regulatory T-cells. Expression of immune response-related proteins from many immune-cell types might be suppressed by Mtb infection. Our study has provided a better insight into the host-pathogen interaction and immune response against different Mtb lineages.

Funder

Research and Diagnostic Center for Emerging Infectious Diseases

Khon Kaen University, Thailand

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Reference48 articles.

1. The role of Mycobacterium tuberculosis lineages on lung tissue damage and TNF-α level among tuberculosis patients, Indonesia;Amin;Clinical Epidemiology and Global Health,2019

2. The success and failure of BCG - implications for a novel tuberculosis vaccine;Andersen;Nature Reviews Microbiology,2005

3. RNF144b is a negative regulator in TLR2-mediated NF-kB activation;Baek;The Journal of Immunology,2016

4. Mycobacterium tuberculosis Inhibits Autocrine Type I IFN signaling to increase intracellular survival;Banks;The Journal of Immunology,2019

5. Old and new selective pressures on Mycobacterium tuberculosis;Brites;Infection, Genetics and Evolution,2012

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