Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

Author:

Wan Jin-Yi1,Wan Jing-Xuan1,Wang Shilei2,Wang Xiaolu1,Guo Wenqian1,Ma Han1,Wu Yuqi1ORCID,Wang Chong-Zhi3,Qi Lian-Wen2,Li Ping2,Yao Haiqiang1,Yuan Chun-Su3

Affiliation:

1. School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China

2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China

3. Tang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA

Abstract

Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.

Funder

National Natural Science Foundation of China

Young Talent Promotion Project of China Association of Chinese Medicine

Young Scientists Development Program of Beijing University of Chinese Medicine, Fundamental Research Funds for the Central Universities

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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