Identification and quantification of flavonoids in 207 cultivated lotus (Nelumbo nucifera) and their contribution to different colors

Abstract

Sacred lotus (Nelumbo nucifera) is a large economic crop, which is also cultivated as a horticultural crop. This study performed a systematic qualitative and quantitative determination of five anthocyanins and 18 non-anthocyanin flavonoids from the petals of 207 lotus cultivars. Among the compounds identified in this study, quercetin 3-O-pentose-glucuronide, quercetin 7-O-glucoside, laricitrin 3-O-hexose, and laricitrin 3-O-glucuronide were discovered for the first time in sacred lotus. The relationships between these pigments and petals colors were also evaluated. A decrease in the total content of anthocyanins and increase in the content of myricetin 3-O-glucuronide resulted in a lighter flower color. Furthermore, petals were yellow when the content of quercetin 3-O-neohesperidoside and myricetin 3-O-glucuronide were increased, whereas petals were red when the total anthocyanin content was high and the quercetin 3-O-sambubioside content was low. These investigations contribute to the understanding of mechanisms that underlie the development of flower color and provide a solid theoretical basis for the further study of sacred lotus.