Rapid response to anthropogenic climate change byThuja occidentalis: implications for past climate reconstructions and future climate predictions

Abstract

Carbon isotope values of leaves (δ13Cleaf) from meta-analyses and growth chamber studies of C3plants have been used to propose generalized relationships between δ13Cleafand climate variables such as mean annual precipitation (MAP), atmospheric concentration of carbon dioxide ([CO2]), and other climate variables. These generalized relationships are frequently applied to the fossil record to create paleoclimate reconstructions. Although plant evolution influences biochemistry and response to environmental stress, few studies have assessed species-specific carbon assimilation as it relates to climate outside of a laboratory. We measured δ13Cleafvalues and C:N ratios of a wide-ranging evergreen conifer with a long fossil record,Thuja occidentalis(Cupressaceae) collected 1804–2017, in order to maximize potential paleo-applications of our focal species. This high-resolution record represents a natural experiment from pre-Industrial to Industrial times, which spans a range of geologically meaningful [CO2] and δ13Catmvalues. Δleafvalues (carbon isotope discrimination between δ13Catmand δ13Cleaf) remain constant across climate conditions, indicating limited response to environmental stress. Only δ13Cleafand δ13Catmvalues showed a strong relationship (linear), thus, δ13Cleafis an excellent record of carbon isotopic changes in the atmosphere during Industrialization. In contrast with previous free-air concentration enrichment experiments, no relationship was found between C:N ratios and increasing [CO2]. Simultaneously static C:N ratios and Δleafin light of increasing CO2highlights plants’ inability to match rapid climate change with increased carbon assimilation as previously expected; Δleafvalues are not reliable tools to reconstruct MAP and [CO2], and δ13Cleafvalues only decrease with [CO2] in line with atmospheric carbon isotope changes.