Population structure and diversification of Gymnospermium kiangnanense, a plant species with extremely small populations endemic to eastern China

Author:

Liu Xiangnan1,Wang Meizhen2ORCID,Song Shiqiang1,Ma Qing3,Yang Zhaoping1ORCID

Affiliation:

1. College of Life Sciences and Technology, Tarim University, Alar, Xinjiang, China

2. College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China

3. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, Zhejiang, China

Abstract

Background Gymnospermium kiangnanense is the only species distributed in the subtropical region within the spring ephemeral genus Gymnospermium. Extensive human exploitation and habitat destruction have resulted in a rapid shrink of G. kiangnanense populations. This study utilizes microsatellite markers to analyze the genetic diversity and structure and to deduce historical population events of extant populations of G. kiangnanense. Methods A total of 143 individuals from eight extant populations of G. kiangnanense, including two populations from Anhui Province and six populations from Zhejiang Province, were analyzed with using 21 pairs of microsatellite markers. Genetic diversity indices were calculated using Cervus, GENEPOP, GenALEX. Population structure was assessed using genetic distance (UPGMA), principal coordinate analysis (PCoA), Bayesian clustering method (STRUCTURE), and molecular variation analysis of variance (AMOVA). Population history events were inferred using DIYABC. Results The studied populations of G. kiangnanense exhibited a low level of genetic diversity (He = 0.179, I = 0.286), but a high degree of genetic differentiation (FST = 0.521). The mean value of gene flow (Nm) among populations was 1.082, indicating prevalent gene exchange via pollen dispersal. Phylogeographic analyses suggested that the populations of G. kiangnanense were divided into two lineages, Zhejiang (ZJ) and Anhui (AH). These two lineages were separated by the Huangshan-Tianmu Mountain Range. AMOVA analysis revealed that 36.59% of total genetic variation occurred between the two groups. The ZJ lineage was further divided into the Hangzhou (ZJH) and Zhuji (ZJZ) lineages, separated by the Longmen Mountain and Fuchun River. DIYABC analyses suggested that the ZJ and AH lineages were separated at 5.592 ka, likely due to the impact of Holocene climate change and human activities. Subsequently, the ZJZ lineage diverged from the ZJH lineage around 2.112 ka. Given the limited distribution of G. kiangnanense and the significant genetic differentiation among its lineages, both in-situ and ex-situ conservation strategies should be implemented to protect the germplasm resources of G. kiangnanense.

Funder

National Natural Science Foundation of China

Zhejiang Provincial Public Welfare Technology and Application Research Project

Shanghai Municipal Administration of Forestation and City Appearances

Publisher

PeerJ

Reference36 articles.

1. Anhui Provincial People’s Government on the publication of the provincial key protection of wild plants list notice;Anhui Provincial People’s Government,2022

2. Molecular characterization of Dendrobium nobile Lindl., an endangered medicinal orchid, based on randomly amplified polymorphic DNA;Bhattacharyya;Plant Systematics and Evolution,2015

3. Ecological consequences of human niche construction: examining long-term anthropogenic shaping of global species distributions;Boivin;Proceedings of the National Academy of Sciences of the United States of America,2016

4. Holocene vegetation history with implications of human impact in the Lake Chaohu area, Anhui Province, East China;Chen;Vegetation History and Archaeobotany,2009

5. Plantae novae ex provincia Zhejiang (Chekiang);Chiu;Journal of University of Chinese Academy of Sciences,1980

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