Sucrose rinse modulates the salivary behavior of carbonic anhydrase VI and its buffering capacity: a longitudinal study in 4 to 6.5-year-old children

Author:

Souza Thayse Rodrigues de1,Zancope Bruna Raquel1,de Sousa Emerson Tavares1,Parisotto Thais Manzano2,Rocha Marques Marcelo3,Nobre dos Santos Marinês1

Affiliation:

1. Department of Health Sciences and Pediatric Dentistry, Piracicaba Dental School, Universidade de Campinas, Piracicaba, São Paulo, Brazil

2. Department of Microbiology and Molecular Biology, São Francisco University Dental School, Bragança Paulista, São Paulo, Brazil

3. Department of Morphology, Piracicaba Dental School, Universidade de Campinas, Piracicaba, São Paulo, Brazil

Abstract

Background Carbonic anhydrase VI (CA VI) is crucial in regulating oral pH and predicting susceptibility to dental caries. The hypothesis posits that caries activity may alter the CA VI function, diminishing its capacity to regulate pH effectively and potentially exacerbating cariogenic challenges. This 1-year cohort study sought to investigate the enzymatic activity of salivary CA VI and buffering capacity following a 20% sucrose rinse in 4 to 6.5-year-old children. Method This research involved 46 volunteers categorized into three groups based on their caries status after follow-up: caries-free (CFee), arrested caries (CArrested), and caries active (CActive). Children underwent visible biofilm examination and saliva collection for salivary flow rate, buffering capacity, and CA VI analyses before and after a 20% sucrose rinse. Results A reduction in the buffering capacity was observed after sucrose rinse in all groups. The CA VI activity decreased significantly in CFee and CArrested groups after sucrose rinse, although it did not change in the CActive group. An improvement in the buffering capacity and salivary flow rate was found at follow-up when compared with the baseline. After 1-year follow-up, buffering capacity and salivary flow rate increased in all groups, whilst the CA VI activity reduced only in CFree and CArrested children. Conclusion Sucrose rinse universally reduces the salivary buffering capacity, while caries activity may disrupt CA VI activity response during a cariogenic challenge. After a year, increased salivary flow enhances buffering capacity but not CA VI activity in caries-active children.

Funder

São Paulo Research Foundation

Publisher

PeerJ

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