A DNA barcode survey of insect biodiversity in Pakistan

Author:

Ashfaq Muhammad1,Khan Arif M.2,Rasool Akhtar3,Akhtar Saleem4,Nazir Naila5,Ahmed Nazeer6,Manzoor Farkhanda7,Sones Jayme8,Perez Kate8,Sarwar Ghulam9,Khan Azhar A.10,Akhter Muhammad11,Saeed Shafqat12,Sultana Riffat13,Tahir Hafiz Muhammad14,Rafi Muhammad A.15,Iftikhar Romana16,Naseem Muhammad Tayyib17,Masood Mariyam18,Tufail Muhammad19,Kumar Santosh20,Afzal Sabila21,McKeown Jaclyn8,Samejo Ahmed Ali13,Khaliq Imran19,D’Souza Michelle L.8,Mansoor Shahid22,Hebert Paul D. N.1ORCID

Affiliation:

1. Centre for Biodiversity Genomics & Department of Integrative Biology, University of Guelph, Guelph, Canada

2. Department of Biotechnology, University of Sargodha, Sargodha, Pakistan

3. Centre for Animal Sciences and Fisheries, University of Swat, Mingora, Pakistan

4. Directorate of Entomology, Ayub Agricultural Research Institute, Faisalabad, Pakistan

5. Department of Entomology, University of Poonch, Rawalakot, Azad Kashmir, Pakistan

6. Faculty of Life Sciences and Informatics, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan

7. Department of Zoology, Lahore College for Women University, Lahore, Pakistan

8. Centre for Biodiversity Genomics, University of Guelph, Guelph, Canada

9. Institute of Zoology, University of the Punjab, Lahore, Pakistan

10. College of Agriculture, Bahauddin Zakariya University Bahadur Campus, Layyah, Pakistan

11. Pulses Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan

12. Faculty of Agriculture and Environmental Sciences, MNS University of Agriculture, Multan, Pakistan

13. Department of Zoology, University of Sindh, Jamshoro, Pakistan

14. Department of Zoology, Government College University Lahore, Lahore, Pakistan

15. National Insect Museum, National Agricultural Research Center, Islamabad, Pakistan

16. Department of Plant Pathology, Washington State University, Pullman, WA, United States

17. Department of Biology, University of Copenhagen, Copenhagen, Denmark

18. Government College Women University Faisalabad, Faisalabad, Pakistan

19. Ghazi University, Dera Ghazi Khan, Pakistan

20. Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan

21. Department of Zoology, University of Narowal, Narowal, Pakistan

22. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

Abstract

Although Pakistan has rich biodiversity, many groups are poorly known, particularly insects. To address this gap, we employed DNA barcoding to survey its insect diversity. Specimens obtained through diverse collecting methods at 1,858 sites across Pakistan from 2010–2019 were examined for sequence variation in the 658 bp barcode region of the cytochrome c oxidase 1 (COI) gene. Sequences from nearly 49,000 specimens were assigned to 6,590 Barcode Index Numbers (BINs), a proxy for species, and most (88%) also possessed a representative image on the Barcode of Life Data System (BOLD). By coupling morphological inspections with barcode matches on BOLD, every BIN was assigned to an order (19) and most (99.8%) were placed to a family (362). However, just 40% of the BINs were assigned to a genus (1,375) and 21% to a species (1,364). Five orders (Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera) accounted for 92% of the specimens and BINs. More than half of the BINs (59%) are so far only known from Pakistan, but others have also been reported from Bangladesh (13%), India (12%), and China (8%). Representing the first DNA barcode survey of the insect fauna in any South Asian country, this study provides the foundation for a complete inventory of the insect fauna in Pakistan while also contributing to the global DNA barcode reference library.

Funder

International Development Research Centre (IDRC) in Canada

Higher Education Commission of Pakistan

Government of Canada

Canada First Research Excellence Fund

New Frontiers in Research Fund

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Reference72 articles.

1. First report and redescription of five species of genus Myrmeleon (Neuroptra: Myrmeleontidae) from Pakistan;Akhtar;Journal of Biodiversity and Environmental Sciences,2018

2. Metabarcoding of freshwater invertebrates to detect the effects of a pesticide spill;Andújar;Molecular Ecology,2018

3. Biodiversity conservation status in Pakistan-a review;Anwar;Pakistan Journal of Forestry,2008

4. DNA barcode analysis of butterfly species from Pakistan points towards regional endemism;Ashfaq;Molecular Ecology Resources,2013

5. Mapping global biodiversity connections with DNA barcodes: lepidoptera of Pakistan;Ashfaq;PLOS ONE,2017

Cited by 9 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3