Characterization of human tear proteome reveals differentially abundance proteins in thyroid-associated ophthalmopathy

Author:

Zhou Xiaoqing1,Wei Ruili1,Wang Rui2

Affiliation:

1. Department of Ophthalmology, Shanghai Changzheng Hospital, Shanghai, Shanghai, China

2. Proteome Research Cente, Shanghai Applied Protein Technology, Shanghai, Shanghai, China

Abstract

Background Thyroid-associated ophthalmopathy (TAO) is a common orbital inflammatory disease, but the abnormal expression of proteins in tears of TAO patients has not been systematically studied. The purpose of this study is to compare and analyze the total tear protein profile of TAO patients and to provide protein cues for TAO pathogenesis. Methods Tear samples were isolated from 30 TAO patients with obvious ocular surface damage and 30 healthy control subjects. Tear samples from 30 individuals were mixed and divided into three sample pools. Easy nano-scale LC-MS/MS based on labeling-free quantitative technology was utilized to profile tear proteome. Results Here, electrospray ionization mass spectra and SDS-PAGE results confirmed the good parallelisms among samples. A total of 313 proteins were obtained from six tear pools, among them, 103 differential abundance proteins (DAPs) were identified, including 99 up-regulated DAPs (including APOA1, HV103, IGH, and Transferrin variant) and four down-regulated DAPs (including FABA, VCC1, NUCB2, and E-cadherin) in the TAO group compared with the control group. GO analysis showed that up-regulated DAPs were mainly enriched in lipid metabolism and platelet molecular function, and down-regulated DAPs were involved in binding, cell junction, and cellular process. KEGG results indicated that DAPs were involved in 117 kinds of signal transduction pathways, among which the immune-related pathway of complement and coagulation cascades had the greatest relevance. Conclusion In conclusion, label-free LC-MS/MS is an effective strategy for profiling tear proteins component. Our study provides proteins and pathways altered in TAO and provides protein cues for further study on the precise mechanism of TAO pathogenesis.

Funder

Changzheng Hospital Youth Fund, Shanghai, China

National Natural Science Foundation of China

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Reference37 articles.

1. Wortmannin, PI3K/Akt signaling pathway inhibitor, attenuates thyroid injury associated with severe acute pancreatitis in rats;Abliz;International Journal of Clinical and Experimental Pathology,2015

2. Natural immunoglobulin M-based delivery of a complement alternative pathway inhibitor in mouse models of retinal degeneration;Annamalai;Experimental Eye Research,2021

3. IgG, IgA and C3 deposits in the extra-thyroidal manifestations of autoimmune Graves’ disease: their in vitro solubilization by intravenous immunoglobulin;Antonelli;Clinical and Experimental Rheumatology,1996

4. Graves’ ophthalmopathy;Bahn;New England Journal of Medicine,2010

5. Altered tear composition in smokers and patients with graves ophthalmopathy;Baker;Archives of Ophthalmology,2006

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