Phenotypic and microRNA transcriptomic profiling of the MDA-MB-231 spheroid-enriched CSCs with comparison of MCF-7 microRNA profiling dataset

Author:

Boo Lily1,Ho Wan Yong2,Mohd Ali Norlaily1,Yeap Swee Keong3,Ky Huynh4,Chan Kok Gan5,Yin Wai Fong5,Satharasinghe Dilan Amila6,Liew Woan Charn7,Tan Sheau Wei7,Cheong Soon Keng18,Ong Han Kiat1

Affiliation:

1. Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Cheras, Selangor, Malaysia

2. Department of Biomedical Sciences, University of Nottingham, Semenyih, Selangor, Malaysia

3. Xiamen University Malaysia, Sepang, Selangor, Malaysia

4. Department of Genetics and Plant Breeding, Cantho University, Cantho, Vietnam

5. Division of Genetics and Molecular Biology, University of Malaya, Kuala Lumpur, Malaysia

6. Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Central, Sri Lanka

7. Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia

8. Cryocord Sdn Bhd, Cyberjaya, Selangor, Malaysia

Abstract

Breast cancer spheroids have been widely used as in vitro models of cancer stem cells (CSCs), yet little is known about their phenotypic characteristics and microRNAs (miRNAs) expression profiles. The objectives of this research were to evaluate the phenotypic characteristics of MDA-MB-231 spheroid-enriched cells for their CSCs properties and also to determine their miRNAs expression profile. Similar to our previously published MCF-7 spheroid, MDA-MB-231 spheroid also showed typical CSCs characteristics namely self-renewability, expression of putative CSCs-related surface markers and enhancement of drug resistance. From the miRNA profile, miR-15b, miR-34a, miR-148a, miR-628 and miR-196b were shown to be involved in CSCs-associated signalling pathways in both models of spheroids, which highlights the involvement of these miRNAs in maintaining the CSCs features. In addition, unique clusters of miRNAs namely miR-205, miR-181a and miR-204 were found in basal-like spheroid whereas miR-125, miR-760, miR-30c and miR-136 were identified in luminal-like spheroid. Our results highlight the roles of miRNAs as well as novel perspectives of the relevant pathways underlying spheroid-enriched CSCs in breast cancer.

Funder

Ministry of Science, Technology and Innovation of Malaysia (MOSTI)

UM High Impact Research Grants

Universiti Tunku Abdul Rahman Research Fund (UTARRF)

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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