Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma-Reference-Cited by-同舟云学术

Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

Published:2023-01-13 Issue: Volume:11 Page:e14349
ISSN:2167-8359
Container-title:PeerJ
language:en
Short-container-title:

Author:

Martins Balbinot Karolyny¹,Almeida Loureiro Felippe José²,Chemelo Giordanna Pereira²,Alves Mesquita Ricardo³^ORCID,Cruz Ramos Aline Maria Pereira⁴^ORCID,Ramos Rommel Thiago Jucá⁵^ORCID,da Costa da Silva Artur Luiz⁵^ORCID,de Menezes Sílvio Augusto Fernandes⁶,da Silva Kataoka Maria Sueli²,Alves Junior Sergio de Melo¹,Viana Pinheiro João de Jesus²^ORCID

Affiliation:

1. Laboratory of Pathological Anatomy and Immunohistochemistry, Federal University of Pará, Belém, Pará, Brazil

2. Cell Cultivation Laboratory, Federal University of Pará, Belém, Pará, Brazil

3. Department of Oral Surgery and Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

4. Health Science Institute, Federal University of Pará, Belém, Pará, Brazil

5. Biological Engineer Laboratory, Park of Science and Technology, Belém, Pará, Brazil

6. Department of Periodontics, Pará State University, Belém, Pará, Brazil

Abstract

Background Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. Methodology To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. Results Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). Conclusions The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.

Funder

National Council for Scientific and Technological Development, CNPq

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Link

https://peerj.com/articles/14349.pdf

Reference33 articles.

1. Multipotent cell lineages in early mouse development depend on SOX2 function;Avilion;Genes & Development,2003

2. SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma;Boumahdi;Nature,2014

3. Core transcriptional regulatory circuitry in human embryonic stem cells;Boyer;Cell,2005

4. Expression of stem cell markers in stroma of odontogenic cysts and tumors;Chacham;Journal of Oral Pathology & Medicine: Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,2020

5. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells;Chambers;Cell,2003

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis;Genes;2023-08-30

2. Expression of stem cell markers SALL4, LIN28A, and KLF4 in ameloblastoma;Diagnostic Pathology;2023-08-09

3. Potential involvement of polycystins in the pathogenesis of ameloblastomas: Analysis based on bioinformatics and immunohistochemistry;Archives of Oral Biology;2023-05