Affiliation:
1. School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, The People’s Republic of China
2. Guizhou Institution of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, The People’s Republic of China
3. Guizhou Weining plateau Grassland Experimental Station, Weining, Guizhou, The People’s Republic of China
4. College of Animal Science, Guizhou University, Guiyang, Guizhou, The People’s Republic of China
Abstract
Although the effects of herbivore camping on soil physicochemical properties have been studied, whether the effects alter the soil microbial communities (e.g., composition, functions, taxonomic and functional diversities, network) remain unknown, especially below the surface. Here, using paired subsoil samples from half month-camping and non-camping, we showed for the first time that camping significantly changed the relative abundance of 21 bacterial phylotypes and five fungal phylotypes. Specifically, we observed significant increases in the relative abundance of putative chitinase and terpenes vanillin-decomposition genes, nitrite reduction function (nirB, nasA), decreases in the relative abundance of putative carbon fixation genes (ackA, PGK, and Pak), starch-decomposition gene (dexB), gene coding nitrogenase (anfG), and tetracycline resistance gene (tetB) for bacterial communities, and significant decreases in the relative abundance of animal endosymbiont and increases in the relative abundance of litter saprotroph and endophyte for fungal communities. However, camping did not significantly impact the taxonomic and functional diversity. The niche restriction was the main driving force of bacterial and fungal community assembly. Compared to no camping, camping increased the stability of bacterial networks but decreased the stability of fungal networks. Camping exerted a positive effect on the network by compressing the niche width and reduced the change in the network by reducing the niche overlap. Our results suggest that camping restructures the soil microbial composition, function, and network, and provides a novel insight into the effect of animal camping on soil microbial communities in grassland.
Funder
National Nature Science Foundation of China
Technology Foundation of China
Guizhou Province Science
Qiannongkeyuanguojihoubuzhu project
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience
Reference76 articles.
1. Crop-based composting of lignocellulosic digestates: focus on bacterial and fungal diversity;Akyol;Bioresource Technology,2019
2. Microbial community composition and antimicrobial resistance in agricultural soils fertilized with livestock manure from conventional farming in Northern Italy;Andrea;Science of The Total Environment,2021
3. Manure and inorganic fertilization impacts on soil nutrients, aggregate stability, and organic carbon and nitrogen in different aggregate fractions;Asmita;Archives of Agronomy and Soil Science,2021
4. Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots;Banerjee;The ISME Journal,2019
5. Controlling the false discovery rate: a practical and powerful approach to multiple testing;Benjamini;Journal of the Royal Statistical Society Series B,1995
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献