Identification of quantitative trait loci (QTLs) regulating leaf SPAD value and trichome density in mungbean (Vigna radiata L.) using genotyping-by-sequencing (GBS) approach

Author:

Kumari Nikki1,Mishra Gyan Prakash1,Dikshit Harsh Kumar1,Gupta Soma1,Roy Anirban2,Sinha Subodh Kumar3,Mishra Dwijesh C.4,Das Shouvik1,Kumar Ranjeet R.5,Nair Ramakrishnan Madhavan6,Aski Muraleedhar1

Affiliation:

1. Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India

2. Plant Pathology, Indian Agricultural Research Institute, New Delhi, Delhi, India

3. Biotechnology, National Institute of Plant Biotechnology, New Delhi, Delhi, India

4. Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute, New Delhi, Delhi, India

5. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, Delhi, India

6. ICRISAT, World Vegetable Center, South Asia, ICRISAT Campus, Patancheru, Hyderabad, India

Abstract

Quantitative trait loci (QTL) mapping is used for the precise localization of genomic regions regulating various traits in plants. Two major QTLs regulating Soil Plant Analysis Development (SPAD) value (qSPAD-7-1) and trichome density (qTric-7-2) in mungbean were identified using recombinant inbred line (RIL) populations (PMR-1×Pusa Baisakhi) on chromosome 7. Functional analysis of QTL region identified 35 candidate genes for SPAD value (16 No) and trichome (19 No) traits. The candidate genes regulating trichome density on the dorsal leaf surface of the mungbean include VRADI07G24840, VRADI07G17780, and VRADI07G15650, which encodes for ZFP6, TFs bHLH DNA-binding superfamily protein, and MYB102, respectively. Also, candidate genes having vital roles in chlorophyll biosynthesis are VRADIO7G29860, VRADIO7G29450, and VRADIO7G28520, which encodes for s-adenosyl-L-methionine, FTSHI1 protein, and CRS2-associated factor, respectively. The findings unfolded the opportunity for the development of customized genotypes having high SPAD value and high trichome density having a possible role in yield and mungbean yellow vein mosaic India virus (MYMIV) resistance in mungbean.

Funder

Indian Council of Agricultural Research (ICAR), New Delhi

SERB (Science and Engineering Research Board), New Delhi

World Vegetable Center: Taiwan

The United States Agency for International Development

The UK Government’s Foreign, Commonwealth & Development Office

Australian Centre for International Agricultural Research

ACIAR Project on International Mungbean Improvement Network

Publisher

PeerJ

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