In vitro and ex vitro propagation of Turkish myrtles through conventional and plantform bioreactor systems

Author:

Şimşek Özhan1,Dönmez Dicle2,Sarıdaş Mehmet Ali3,Acar Emine4,Aka Kaçar Yıldız3,Paydaş Kargı Sevgi3,İzgü Tolga5

Affiliation:

1. Department of Horticulture, Erciyes University, Kayseri, Türkiye

2. Biotechnology Research and Application Center, University of Çukurova, Adana, Türkiye

3. Horticulture Department, University of Çukurova, Adana, Türkiye

4. Biotechnology Department, Institute of Applied and Natural Sciences, University of Çukurova, Adana, Türkiye

5. National Research Council of Italy (CNR), IBE/Institute of BioEconomy, Florence, Italy

Abstract

The myrtle (Myrtus communis) plant naturally grows in the temperate Mediterranean and subtropical regions and is used for various purposes; thus, it is among the promising species of horticultural crops. This study aimed to evaluate and compare the performance of different propagation systems, including rooting, solid media propagation, rooting, and with the Plantform bioreactor system, in achieving healthy and rapid growth of four myrtle genotypes with diverse genetic origins and well-regional adaptation. The selection of myrtle genotypes with distinct genetic backgrounds and proven adaptability to specific regions allowed for a comprehensive assessment of the propagation systems under investigation. Present findings proved that the Plantform system, the new-generation tissue culture system, was quite successful in micropropagation and rooting myrtle genotypes. We succeeded in vitro micropropagation and rooting of diverse wild myrtle genotypes, enabling year-round propagation without reliance on specific seasons or environmental conditions. The process involved initiating cultures from explants and multiplying them through shoot proliferation in a controlled environment. This contributes to sustainable plant propagation, preserving and utilizing genetic resources for conservation and agriculture.

Funder

TÜBİTAK

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Reference35 articles.

1. Evaluation and comparison of a new type of temporary immersion system (TIS) bioreactors for myrtle (Myrtus communis L.);Aka Kaçar;Applied Ecology and Environmental Research,2020

2. Comparison of methods of liquid medium culture for banana micropropagation;Alvard;Plant Cell, Tissue and Organ Culture,1993

3. Plant Form. A Temporary immersion system, for in vitro propagation of M. communis and Olea europae;Benelli,2015

4. Micropropagation and rooting of myrtle plant (Myrtus communis L.) by classical and next generation plant tissue culture tecniques;Biçen;MSc thesis,2017

5. Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae);Canhoto;Plant Cell, Tissue and Organ Culture,1999

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