Urine proteomic analysis of the rat e-cigarette model

Author:

Liu Yuqing1,Shen Ziyun1,Zhao Chenyang1,Gao Youhe1

Affiliation:

1. Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, China

Abstract

Background We were curious if the urinary proteome could reflect the effects of e-cigarettes on the organism. Methods Urine samples were collected from a rat e-cigarette model before, during, and after two weeks of e-cigarette smoking. Urine proteomes before and after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. Results Fetuin-B, a biomarker of chronic obstructive pulmonary disease (COPD), and annexin A2, which is recognized as a multiple tumour marker, were identified as differential proteins in five out of six smoking rats on day 3. To our surprise, odourant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated. Pathways enriched by the differential proteins include the apelin signalling pathway, folate biosynthesis pathway, arachidonic acid metabolism, chemical carcinogenesis-DNA adducts and chemical carcinogenesis-reactive oxygen species. They have been reported to be associated with immune system, cardiovascular system, respiratory system, etc. Conclusions Urinary proteome could reflect the effects of e-cigarettes in rats.

Funder

The National Key Research and Development Program of China

The Fundamental Research Funds for the Central Universities

The Beijing Natural Science Foundation

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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