EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY

Abstract

This study aimed to examine the effects of albumin administration on ischemia-reperfusion in the rat ovary by using biochemical, histological, and immunohistochemical methods. Thirty-two Wistar albino rats were used in the study, and they were divided into 4 groups: control, albumin, placebo, and ischemia-reperfusion. Healthy ovaries were taken from the first group. In the other three groups, 2-hour ischemia and 2-hour reperfusion were applied to the bilateral ovaries. In the albumin group, intraperitoneal albumin (2.5 g/kg, 20% human albumin) was administered 30 minutes before reperfusion, and in the placebo group, the same volume of intraperitoneal saline was administered instead of albumin 30 minutes before reperfusion. Ovarian damage scores, cytochrome C-1 immunoreactivity, total oxidant status, total antioxidant status, and oxidative stress index levels were evaluated. In the statistical analysis performed between the groups, it was seen that the results of the control group were significantly lower than the ischemia-reperfusion group in terms of total oxidant status values (p=0.001), and the results of the ischemia-reperfusion group were significantly higher than the control and albumin groups in terms of oxidative stress index values (p<0.001 and p=0.004, respectively). In histological examinations, the total damage score obtained by evaluating follicular degeneration, edema, vascular congestion, and hemorrhage was found to be significantly higher in the ischemia-reperfusion group than in the control group (p=0.003). According to the immunohistochemical examination results, cytochrome C-1 immunoreactivity in the ischemia-reperfusion group was significantly stronger than the control and albumin groups (p<0.001). We think that albumin administration reduces cytochrome C-1, reactive oxygen species, and oxidative stress levels, therefore it will play a helpful role in the ischemia-reperfusion treatment process.