Isolation and Quantification of Scopoletin from Leaves and Marketed Formulation of Morinda Citrifolia L.

Abstract

Objective This study aims to isolate and quantify Scopoletin in leaves and commercial noni juice products of Morinda citrifolia. Methods TLC and HPTLC methods were developed for the isolation and quantification of Scopoletin. Results The ethanol leaf extract of Morinda citrifolia contains 7.4 mg of Scopoletin per 1 g of extract. Noni juices A, B, and C were found to contain 41 mg, 25.7 mg, and 60.93 mg of Scopoletin per 100 ml, respectively. The quantity of Scopoletin in different brands of noni juices may vary due to changes in temperature, season, and manufacturing processes. Noni juice sample D did not show the presence of Scopoletin, possibly due to its combination with Aloe vera and Garcinia cambogia. Conclusion The results indicate that the ethanolic extract of noni leaf and commercial noni juice products contain the marker compound Scopoletin. Further in vitro and in vivo experiments are needed to explore its mechanism of action and therapeutic properties.